Plenary presentation by G. Volpe at the MüSIM, Münster, Germany, 24 June 2026.

Illustration of three different experiments autonomously performed by the SmartTrap system: DNA pulling experiments (top), red blood cell stretching (bottom left), and particle-particle interaction measurements (bottom right). (Image by M. Selin.)
Smart Machines and Optical Manipulation at the Microscale
Giovanni Volpe
5th Münster Symposium on Intelligent Matter (MüSIM) (Flyer)
Date: 24 June 2026
Time: 15:00
Place: Center for Soft Nanoscience (SoN), Münster, Germany

Microscale systems offer a unique opportunity to engineer machines whose function emerges from the interplay of geometry, interactions, and fluctuations. In this talk, I will present our work on smart machines at the microscale, combining nanofabrication, programmable interactions, and advanced optical methods to design and control colloidal micromechanisms and metamaterials.
I will first introduce how nanotechnology enables the realization of colloidal metamachines and microscopic mechanisms, where shape and mechanical constraints are engineered to produce targeted motion and response in fluid environments. I will then show how smart microscopy and optical manipulation—including high-resolution imaging, automated tracking, and light-based control—allow us to probe these machines in real time and quantify their dynamics. This approach enables precision measurements of effective interaction landscapes, including critical Casimir forces and their relation to fluctuation-induced forces known from QED Casimir physics.

Invited Seminar by G. Volpe at QT community building activity, Stenungsbaden Yacht club, 11 May 2026

(Image created by G. Volpe with the assistance of DALL·E 2)
What remain for physicists to do in the age of AI?
Giovanni Volpe
QT (Quantum Technology Division of MC2, Chalmers University of Technology) community building activity 2026
Date: 11 May 2026
Place: Stenungsbaden Yacht club

In recent years, the rapid growth of artificial intelligence, particularly deep learning, has transformed fields from natural sciences to technology. While deep learning is often viewed as a glorified form of curve fitting, its advancement to multi-layered, deep neural networks has resulted in unprecedented performance improvements, often surprising experts. As AI models grow larger and more complex, many wonder whether AI will eventually take over the world and what role remains for physicists and, more broadly, humans.

A critical, yet underappreciated fact is that these AI systems rely heavily on vast amounts of training data, most of which are generated and annotated by humans. This dependency raises an intriguing issue: what happens when human-generated data is no longer available, or when AI begins to train on AI-generated data? The phenomenon of AI poisoning, where the quality of AI outputs declines due to self-referencing, demonstrates the limitations of current AI models. For example, in image recognition tasks, such as those involving the MNIST dataset, AI tends to gravitate towards ‘safe’ or average outputs, diminishing originality and accuracy.

In this context, the unique role of humans becomes clear. Physicists, with their capacity for originality, deep understanding of physical phenomena, and the ability to exploit fundamental symmetries in nature, bring invaluable perspectives to the development of AI. By incorporating physics-informed training architectures and embracing the human drive for meaning and discovery, we can guide the future of AI in truly innovative directions. The message is clear: physicists must remain original, pursue their passions, and continue searching for the hidden laws that govern the world and society.

MSCA-DN SPM4.0 training event in Madrid, 13-17 April 2026

The Madrid Institute of Materials Sciences (CSIC-ICMM) hosted the second training workshop for the SPM 4.0 network. Both Prakhar Dutta and Jiacheng Huang, the two doctoral candidates based at the University of Gothenburg, participated to the event along with the other doctoral candidates of the network.
The second training workshop started with presentations from the doctoral candidates on their progress so far. The training event also consisted of a series of lectures on different topics such as a deep dive into atomic force microscopy and the different modes for the same, basics of deep learning, and an overview of data management plans.
ICMM also hosted some practical sessions where hands-on lectures were given on the use of atomic force microscopy machines and their applications.

Presentation by S. K. Mondal, online, 22 April, 2026

Optical Fiber Micro/Nano Axicon Tip: An Optical Imaging Platform
Samir K. Mondal
CSIR-CSIO, Chandigarh, India
Date: 22 April 2026
Time: 12:30
Place: Online on zoom

Optical fiber tip under structural modifications enhances light-matter interaction by focusing, collecting or modulating light in microscopic scale and combined with waveguide property, it emerges as a potential optical tool, especially for spectroscopic, endoscopic and imaging application. A chemical etching technique has been introduced to permanently modify the tip as Micro/Nano axicon, capable in generating structured beams. The optics of the axicons have been studied in detail and further used in optical imaging experiments, namely phase microscopy, photonic nanojet and nanoscopy. The seminar will highlight first-hand information about the probe and experiments addressing the above-mentioned application.

Short Bio

Dr. Mondal is Chief Scientist at CSIR-CSIO, Chandigarh. He earned his Ph.D. in Electronic Science and M.Sc. in Physics from the University of Calcutta. After postdoctoral research at the University of California, Irvine and the University of Minnesota, he joined Tyndall Research Institute, Ireland.

With over 25 years in optics and photonics, his work spans optical interconnects, photonic crystals, lasers, and fiber instrumentation. He leads research in optical fiber antennas, near-field optics, imaging, and plasmonics, aiming for sustainable photonics platforms.

He collaborates internationally and is known for pioneering micro/nano axicons on fiber tips. He has over 50 publications and serves as an editor and reviewer.

Poster by P. Dutta at the Protein Folding in Real Time Conference, Stockholm, 11th March 2026

A coarse-grained molecular dynamics framework used to simulate plasmid DNA analyzed via atomic force microscopy (AFM). The resulting images are used to train a U-Net for DNA chain and crossing segmentation and classification. (Image by P. Dutta.)
ASAP (AFM Simulation and Analysis Pipeline)
Prakhar Dutta, Jiacheng Huang, Nazli Demirpehlivan, Thomas Catley, Sylvia Whittle, Carlo Manzo, Rahul Nagshi, Rachel Owen, Giovanni Volpe
Date: 11th March 2026
Time: 18:00 – 20:00
Place: Aula Medica, Karolinska Institute, Solna
Conference Protein Folding in Real Time, 11-13 March 2026, Stockholm, Sweden

Abstract: Atomic force microscopy (AFM) resolves biological structure and mechanics at high resolution, but produces vast, heterogeneous datasets that are often noisy and very time-consuming to analyse. Although deep learning could automate quality control, segmentation and feature extraction, adoption is limited by scarce ground-truth training data and high technical barriers for experimentalists. Here we present ASAP, an open-source tutorial and pipeline implemented in DeepTrack to provide a reproducible foundation for AI-enabled AFM. At the protein folding conference, a dual-pathway simulation for DNA, offering both molecular dynamics and rapid, non-MD geometries to generate perfect ground truth for segmentation training was presented. By consolidating simulation and learning into a single modular ecosystem, this work enables users to build upon our pipeline to optimize AFM workflows for more efficient data acquisition and robust processing.

Poster by Anqi Lyu at the Protein Folding in Real Time conference, Stockholm, 11 March 2026

Opposing age trajectories and late-life divergence in protein abundance between conditions. (Image by A. Lyu.)
Age-Dependent Plasma Protein Dynamics in Health and Disease
Anqi Lyu, Maria Jesus Iglesias, Jochen Schwenk, Mathias Uhlén, Jacob Odeberg, Caroline Adiels, Lynn Butler
Date: 11 March 2026
Time: 18:00-20:00
Place: Aula Medica, Karolinska Institute, Solna
Conference Protein Folding in Real Time, 11-13 March 2026, Stockholm, Sweden

Aging is biologically heterogeneous, and chronological age alone cannot explain molecular variability across individuals. Previous studies have shown that disease states can reshape age-associated proteomic trajectories, leading to divergent molecular patterns over time. Here, we explore these dynamics by analyzing age-dependent changes in plasma protein abundance, focusing on differences between case and control conditions during aging. We identify opposing trends and nonlinear transitions, particularly in later life, highlighting critical periods of accelerated molecular change. Beyond descriptive patterns, our analysis emphasizes how disease modifies the underlying structure of aging trajectories, providing insights into the mechanisms of age-related divergence.

Poster by M. Granfors at the Protein Folding in Real Time conference, Stockholm, 11 March 2026

Fluorescence microscopy image of yeast cells, with Hsp104-GFP marking protein aggregates, making them visible as bright spots. (Image by J. Masaryk.)
Machine learning based tracking of protein aggregates in yeast
Mirja Granfors, Jakub Masaryk, Carlo Manzo, Markus Tamas, Giovanni Volpe
Date: 11th March 2026
Time: 18:00 – 20:00
Place: Aula Medica, Karolinska Institute, Solna
Conference Protein Folding in Real Time, 11-13 March 2026, Stockholm, Sweden

Arsenic is a toxic metal linked to serious diseases like cancer and neurodegeneration. One proposed mechanism of toxicity is that arsenic causes proteins to misfold and aggregate inside cells, but the dynamics and regulation of this process remain poorly understood. Using fluorescence microscopy data from living yeast cells, we are developing a machine learning approach to automatically detect, track, and analyze protein aggregate movement over time.

Poster by X. Zhang at at the Protein Folding in Real Time conference, Stockholm, 11 March 2026

Reconstructed field of LCD2–CTPR4-Func1 condensates with LC3 at the sample plane (shown here as the imaginary component of the complex field). The condensates increase in size through Ostwald ripening and recruitment of LC3. (Image by X. Zhang.)
Quantitative Characterization of Biomolecular Condensates Using Off-Axis Holographic Microscopy
Xinwen Zhang, Nora Haanaes, Berenice García Rodríguez, Giovanni Volpe,  Janet Kumita and Daniel Midtvedt
Date: 11 March 2026
Time: 18:00-20:00
Place: Aula Medica, Karolinska Institute, Solna
Conference Protein Folding in Real Time, 11-13 March 2026, Stockholm, Sweden

Biomolecular condensates formed through liquid–liquid phase separation (LLPS) play important roles in cellular organization, yet quantitative and label-free characterization of their physical properties remains challenging. In this work, we apply off-axis holographic microscopy to study a synthetic biomolecular condensate platform based on the LCD2-CTPR protein system. These proteins, composed of modular consensus-designed tetratricopeptide repeat (CTPR) domains fused to intrinsically disordered regions, undergo phase separation under varying salt concentrations. By incorporating short binding motifs such as ATG13 or Func1, the condensates can specifically recruit the autophagy-related protein LC3. Using label-free quantitative phase measurements, we analyze changes in condensate optical radius and refractive index during LC3 recruitment and over time. Our results show measurable variations in condensate size and optical properties, highlighting the sensitivity of these systems to compositional changes. This work demonstrates the applicability of holographic microscopy for quantitative characterization of synthetic biomolecular condensates and provides a framework for studying protein phase separation in a non-invasive manner.

Poster by E. A. Duta Costache at the Protein Folding in Real Time conference, Stockholm, 11 March 2026

Pointwise absolute error plots for the heat equation tested across five architectures. The plots show the mean absolute error achieved by each architecture on a periodic-mode initial condition. Errors are shown on a logarithmic scale. Blue colors indicate smaller errors. (Image by E. A. Duta Costache.)
The optimization autopsy of PINNs
Eduard Duta Costache, Benjamin Girault
Date: 11 March 2026
Time: 18:00-20:00
Place: Aula Medica, Karolinska Institute, Solna
Conference Protein Folding in Real Time, 11-13 March 2026, Stockholm, Sweden

Physics-Informed Neural Networks (PINNs) have emerged as a promising method for solving Partial Differential Equations (PDEs) by combining data-driven learning with physical laws. However, the spectral bias and optimization challenges limit their efficacy. This work investigates these issues and whether the advantages of classical spectral methods translate to the non-convex neural network optimization landscape. We show that gradient imbalance greatly affect learning and we study the Hessian conditioning under different settings. Our results indicate that spectral priors stabilize training, reduce error, and improve parameter efficiency. We also identify that learnable-basis models act as implicit regularizers under sparse sampling.