Digital video microscopy enhanced by deep learning
Sandoghdar Division, Max Planck Institute for the Science of Light, Erlangen, Germany
10 May 2019
Single particle tracking is essential in many branches of science and technology, from the measurement of biomolecular forces to the study of colloidal crystals. Standard methods rely on algorithmic approaches; by fine-tuning several user-defined parameters, these methods can be highly successful at tracking a well-defined kind of particle under low-noise conditions with constant and homogenous illumination. Here, we introduce an alternative data-driven approach based on a convolutional neural network, which we name DeepTrack. We show that DeepTrack outperforms algorithmic approaches, especially in the presence of noise and under poor illumination conditions. We use DeepTrack to track an optically trapped particle under very noisy and unsteady illumination conditions, where standard algorithmic approaches fail. We then demonstrate how DeepTrack can also be used to track multiple particles and non-spherical objects such as bacteria, also at very low signal-to-noise ratios. In order to make DeepTrack readily available for other users, we provide a Python software package, which can be easily personalized and optimized for specific applications.
Measurement of Anomalous Diffusion Using Recurrent Neural Networks
Stefano Bo, Falko Schmidt, Ralf Eichborn & Giovanni Volpe
Anomalous diffusion occurs in many physical and biological phenomena, when the growth of the mean squared displacement (MSD) with time has an exponent different from one. We show that recurrent neural networks (RNN) can efficiently characterize anomalous diffusion by determining the exponent from a single short trajectory, outperforming the standard estimation based on the MSD when the available data points are limited, as is often the case in experiments. Furthermore, the RNN can handle more complex tasks where there are no standard approaches, such as determining the anomalous diffusion exponent from a trajectory sampled at irregular times, and estimating the switching time and anomalous diffusion exponents of an intermittent system that switches between different kinds of anomalous diffusion. We validate our method on experimental data obtained from sub-diffusive colloids trapped in speckle light fields and super-diffusive microswimmers.
Meltem Elitas is visiting from Sabanci University in Istanbul from 1st May until 28th June 2019.
Meltem Elitas is a faculty member at the Mechatronics Program at Sabanci University in Istanbul, Turkey. Her background is Electrical and Mechatronics Engineering; she obtained her doctorate from Bioengineerieng and Biotechnology Department at École Polytechnique Fédérale de Lausanne. She performed her postdoctoral studies at Yale University Biomedical Engineering Department. She has published more than 25 papers and conference proceedings in reputed journals. Her research interests are biomechatronics, cellular heterogeneity, cellular interactions, tumor microenvironment, live cell imaging and development of microfabricated tools for quantitative biology. She is visiting the Soft Matter Lab as part of her ongoing Marie Skłodowska-Curie project.