John Tember
Date: 25 September 2026
Time: 18:00 – 18:15
Place: Largo Ciro Menotti, Pisa, Italy
Modern tissue-clearing and light-sheet microscopy techniques can generate highly detailed three-dimensional images of biological samples, but the increasing size and complexity of these datasets pose significant challenges for efficient visualization, processing and analysis. Interactive computational tools are therefore essential for exploring large volumetric datasets and extracting quantitative information from them.
FIREFLY is being developed as a framework for interactive rendering, exploration and feature extraction of light-sheet microscopy data. The presentation introduced computational approaches for real-time 3D visualization, volumetric data processing and multi-scan reconstruction, together with ongoing work using self-supervised machine learning to improve the resolution of anisotropic microscopy volumes.
The work was presented at Scintille di Scienza: la Ricerca dal Laboratorio alla Piazza – II Edizione, a public outreach event organized by the Department of Pharmacy at the University of Pisa as part of BRIGHT Night 2026, the European Researchers’ Night in Tuscany. The event brought researchers to the city centre of Pisa for short talks, interactive activities and demonstrations aimed at communicating current research to a broad public audience.
The aim of FIREFLY is to develop computational tools that make increasingly large and complex microscopy datasets easier to explore, process and quantitatively analyse.
