Real-time detection of hyperosmotic stress response in optically trapped single yeast cells using Raman microspectroscopy
Gajendra P. Singh, Caitriona M. Creely, Giovanni Volpe, Helga Grötsch & Dmitri Petrov
Analytical Chemistry 77(8), 2564–2568 (2005)
Living cells survive environmentally stressful conditions by initiating a stress response. We monitored changes in the Raman spectra of optically trapped Saccharomyces cerevisiae yeast cell under normal, heat-treated, and hyperosmotic stress conditions. It is shown that when glucose was used to exert hyperosmotic stress, two chemical substancesglycerol and ethanolcan be monitored in real time in a single cell.