Linde Viaene
Date: 11 March 2026
Time: 18.00-20.00
Place: Aula Medica, Stockholm Sweden
Conference Protein Folding in Real Time, 11-13 March 2026, Stockholm, Sweden
Cell populations are inherently diverse, and averaging measurements across them can mask subtle or rare cellular behaviours. In this work, we use live Slimfield single-molecule microscopy to study the role of Hsp104 in clearing misfolded and aggregated proteins after stress. By analysing endogenously tagged Hsp104, we quantify molecular diffusion and stoichiometry before and after heat stress. Our results show a transition from faster, more mobile molecules to larger, more static assemblies following stress, consistent with Hsp104 functionally engaging with protein aggregates. These measurements provide molecular-level insight into how cells respond to proteotoxic stress.