Single particle tracking is essential in many branches of science and technology, from the measurement of biomolecular forces to the study of colloidal crystals. Standard methods rely on algorithmic approaches; by fine-tuning several user-defined parameters, these methods can be highly successful at tracking a well-defined kind of particle under low-noise conditions with constant and homogenous illumination. Here, we introduce an alternative data-driven approach based on a convolutional neural network, which we name DeepTrack. We show that DeepTrack outperforms algorithmic approaches, especially in the presence of noise and under poor illumination conditions. We use DeepTrack to track an optically trapped particle under very noisy and unsteady illumination conditions, where standard algorithmic approaches fail. We then demonstrate how DeepTrack can also be used to track multiple particles and non-spherical objects such as bacteria, also at very low signal-to-noise ratios. In order to make DeepTrack readily available for other users, we provide a Python software package, which can be easily personalized and optimized for specific applications.
Saga Helgadottir joins the Soft Matter Lab on 28 November 2017 as a PhD student at the Physics Department of the University of Gothenburg.
She has a Master degree in Physics from Chalmers University of Technology with a Master thesis on the study of the effect of plasma on biofilms.
She will work on he PhD thesis on the realisation of hybrid microswimmers and the study of bacterial dynamics in complex and crowded environments.