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Seminar by W. Ahmed on 13 March 2024

A schematic of a passive particle immersed in an active bath experiencing non-equilibrium fluctuations. (Illustration by W. Ahmed)
Emergent behavior in active biological matter
Wylie Ahmed
Laboratoire de Physique Theorique, Toulouse (France) and California State University, Fullerton (USA)

13 March 2024, 12:30, Nexus

Motivated by nucleus centering in mouse oocytes, we explore a different type of biological active matter. We investigate the stochastic force fluctuations of micro swimmers in two scenarios: (1) a single swimmer navigating through a passive fluid; (2) a dense suspension of swimmers surrounding a passive tracer. By direct force measurement using optical tweezers we show that the force trajectory of an individual micro swimmer exhibits rich oscillatory dynamics that vary in time. Interestingly, when these highly fluctuating force dynamics are analyzed using the framework of stochastic thermodynamics we recover energy dissipation rates in agreement with time-averaged fluid dynamics studies. For a dense suspension of swimmers serving as an active bath for a passive tracer we observe both shear thinning and thickening, which depends on Peclet number, and enhanced diffusion of our tracer by a factor of 2. We estimate the energy transfer rate from the active bath to the passive tracer. These two scenarios allow us to explore energy exchange between an active swimmer in a passive bath and a passive tracer in an active bath.

Berenice García Rodríguez presented her half-time seminar on 8 March 2024

Berenice García Rodríguez (right) and opponent Dr. Hana Jungová (left). (Photo by J. P. Ramírez)
Berenice García Rodríguez completed the first half of her doctoral studies, and she defended her half-time on the 8th of March 2024.

The presentation, “Quantitative Analysis of Nanoparticle Properties Using Optical Scattering Techniques,” was held in a hybrid format, with part of the audience in the Nexus room and the rest connected through Zoom. The half-time consisted of a presentation about her past and planned projects, followed by a discussion and questions proposed by her opponent, Dr. Hana Jungová.

The presentation started with a short background introduction to optical scattering techniques and nanoparticle characterization techniques, followed by an introduction and description of the first paper, “Dual-Angle Interferometric Scattering Microscopy for Optical Multiparametric Particle Characterization,” and, in the end, a brief description of the projects in which Berenice is involved.

In the last section, she outlined the proposed continuation of her PhD: quantification and characterization of biomolecular condensates and their evolution over time, monitoring lipid droplets during long timescales inside living cells, and parametrization for core-shell particles.

Giovanni Volpe awarded the Göran Gustafsson prize

(Photo by Johan Wingborg.)
Giovanni Volpe was awarded one of Sweden’s most prestigious prizes for physics, the Göran Gustafsson Prize, which is handed out by the Göran Gustafsson Foundation with the help of the Royal Swedish Academy of Sciences. Giovanni receives the physics prize “for boundary breaking research focusing on microscopic particles with active functions”. The prize sum is 6.3 million SEK.

More details here:
Press release of Gothenburg University: Giovanni Volpe receives prestigious Göran Gustafsson prize
Press release of Kungl. Vetenskapsakademien: 33 miljoner till forskning om bland annat TBE och smarta mikropartiklar

Wylie Ahmed visits the Soft Matter Lab. Welcome!

Wylie Ahmed is a Visiting Professor from the Laboratoire de Physique Theorique in Toulouse, France. He is also an associate professor (on leave) at California State University, Fullerton where he leads the Laboratory for Soft, Living, and Active Matter (SLAMLab). His visiting position is financed through the CNRS with partial support from the Soft Matter Lab.
He will visit us for 5 months from March 1, 2024, to July 31, 2024.

He completed his Ph.D. at the University of Illinois at Urbana-Champaign, and was a Marie Skłodowska-Curie Research Fellow at the Institut Curie in Paris, France. He started his group in 2016 in California and is now moving his research activities to Toulouse France. His research interests are in cellular biophysics, soft and active matter physics, and bio-inspired materials with a theme towards understanding emergent behavior.

Anoop C. Patil joins the Soft Matter Lab

(Photo by Rashmi Anoop Patil.)
Anoop C. Patil joined the Soft Matter lab on March 1, 2023.

Anoop is a Senior Fellow at the Singapore-MIT Alliance for Research & Technology (SMART) center, based in the National University Singapore campus, Singapore.

He is working on computational analysis for precision agriculture at Disruptive & Sustainable Technologies for Agricultural Precision (DiSTAP), SMART, Singapore. As a part of this work, he is also working on the BRAPH-2 platform for spectral analysis applications at DiSTAP, SMART, and Temasek Life Sciences Laboratory (TLL), Singapore.

Dual-Angle Interferometric Scattering Microscopy for Optical Multiparametric Particle Characterization published in Nano Letters

Conceptual schematic of dual-angle interferometric scattering microscopy (DAISY). (Image by the Authors of the manuscript.)
Dual-Angle Interferometric Scattering Microscopy for Optical Multiparametric Particle Characterization
Erik Olsén, Berenice García Rodríguez, Fredrik Skärberg, Petteri Parkkila, Giovanni Volpe, Fredrik Höök, and Daniel Sundås Midtvedt
Nano Letters (2024)
arXiv: 2309.07572
doi: 10.1021/acs.nanolett.3c03539

Traditional single-nanoparticle sizing using optical microscopy techniques assesses size via the diffusion constant, which requires suspended particles to be in a medium of known viscosity. However, these assumptions are typically not fulfilled in complex natural sample environments. Here, we introduce dual-angle interferometric scattering microscopy (DAISY), enabling optical quantification of both size and polarizability of individual nanoparticles (radius <170 nm) without requiring a priori information regarding the surrounding media or super-resolution imaging. DAISY achieves this by combining the information contained in concurrently measured forward and backward scattering images through twilight off-axis holography and interferometric scattering (iSCAT). Going beyond particle size and polarizability, single-particle morphology can be deduced from the fact that the hydrodynamic radius relates to the outer particle radius, while the scattering-based size estimate depends on the internal mass distribution of the particles. We demonstrate this by differentiating biomolecular fractal aggregates from spherical particles in fetal bovine serum at the single-particle level.

Connecting genomic results for psychiatric disorders to human brain cell types and regions reveals convergence with functional connectivity on medRxiv

Brain region connectivity. (Image by the Authors of the manuscript.)
Connecting genomic results for psychiatric disorders to human brain cell types and regions reveals convergence with functional connectivity
Shuyang Yao, Arvid Harder, Fahimeh Darki, Yu-Wei Chang , Ang Li, Kasra Nikouei, Giovanni Volpe, Johan N Lundström, Jian Zeng , Naomi Wray, Yi Lu, Patrick F Sullivan, Jens Hjerling-Leffler
medRxiv: 10.1101/2024.01.18.24301478

Understanding the temporal and spatial brain locations etiological for psychiatric disorders is essential for targeted neurobiological research. Integration of genomic insights from genome-wide association studies with single-cell transcriptomics is a powerful approach although past efforts have necessarily relied on mouse atlases. Leveraging a comprehensive atlas of the adult human brain, we prioritized cell types via the enrichment of SNP-heritabilities for brain diseases, disorders, and traits, progressing from individual cell types to brain regions. Our findings highlight specific neuronal clusters significantly enriched for the SNP-heritabilities for schizophrenia, bipolar disorder, and major depressive disorder along with intelligence, education, and neuroticism. Extrapolation of cell-type results to brain regions reveals important patterns for schizophrenia with distinct subregions in the hippocampus and amygdala exhibiting the highest significance. Cerebral cortical regions display similar enrichments despite the known prefrontal dysfunction in those with schizophrenia highlighting the importance of subcortical connectivity. Using functional MRI connectivity from cases with schizophrenia and neurotypical controls, we identified brain networks that distinguished cases from controls that also confirmed involvement of the central and lateral amygdala, hippocampal body, and prefrontal cortex. Our findings underscore the value of single-cell transcriptomics in decoding the polygenicity of psychiatric disorders and offer a promising convergence of genomic, transcriptomic, and brain imaging modalities toward common biological targets.

Nanoalignment by Critical Casimir Torques on ArXiv

Artist rendition of a disk-shaped microparticle trapped above a circular uncoated pattern within a thin gold layer coated on a glass surface. (Image by the Authors of the manuscript.)
Nanoalignment by Critical Casimir Torques
Gan Wang, Piotr Nowakowski, Nima Farahmand Bafi, Benjamin Midtvedt, Falko Schmidt, Ruggero Verre, Mikael Käll, S. Dietrich, Svyatoslav Kondrat, Giovanni Volpe
arXiv: 2401.06260

The manipulation of microscopic objects requires precise and controllable forces and torques. Recent advances have led to the use of critical Casimir forces as a powerful tool, which can be finely tuned through the temperature of the environment and the chemical properties of the involved objects. For example, these forces have been used to self-organize ensembles of particles and to counteract stiction caused by Casimir-Liftshitz forces. However, until now, the potential of critical Casimir torques has been largely unexplored. Here, we demonstrate that critical Casimir torques can efficiently control the alignment of microscopic objects on nanopatterned substrates. We show experimentally and corroborate with theoretical calculations and Monte Carlo simulations that circular patterns on a substrate can stabilize the position and orientation of microscopic disks. By making the patterns elliptical, such microdisks can be subject to a torque which flips them upright while simultaneously allowing for more accurate control of the microdisk position. More complex patterns can selectively trap 2D-chiral particles and generate particle motion similar to non-equilibrium Brownian ratchets. These findings provide new opportunities for nanotechnological applications requiring precise positioning and orientation of microscopic objects.

Colloquium by G. Volpe at the Mini-Symposium with Giovanni Volpe and Pawel Sikorski, Lund, 11 January 2024

(Image by A. Argun)
Deep Learning for Imaging and Microscopy
Giovanni Volpe
Mini-Symposium with Giovanni Volpe and Pawel Sikorski, Lund, Sweden, 11 January 2024
Date: 11 January 2024
Time: 15:15

Video microscopy has a long history of providing insights and breakthroughs for a broad range of disciplines, from physics to biology. Image analysis to extract quantitative information from video microscopy data has traditionally relied on algorithmic approaches, which are often difficult to implement, time consuming, and computationally expensive. Recently, alternative data-driven approaches using deep learning have greatly improved quantitative digital microscopy, potentially offering automatized, accurate, and fast image analysis. However, the combination of deep learning and video microscopy remains underutilized primarily due to the steep learning curve involved in developing custom deep-learning solutions. To overcome this issue, we have introduced a software, DeepTrack 2.1, to design, train and validate deep-learning solutions for digital microscopy.

Fredrik Skärberg presented his half-time seminar on 10 January 2024

Fredrik Skärberg (right) and opponent Prof. Rebecka Jörnsten (left). (Photo by A. Ciarlo)
Fredrik Skärberg completed the first half of his doctoral studies and he defended his half-time on the 10th of January 2024.

The presentation, with title: “Holographic characterization of biological nanoparticles using deep learning”, was held in hybrid format, with part of the audience in the Nexus room and the rest connected through zoom. The half-time consisted in a presentation about his past and planned projects and it was followed by a discussion and questions proposed by his opponent Prof. Rebecka Jörnsten.

The presentation started with a short background to characterization of biological particles inside cells and an introduction to the papers included in the half-time.

It continued with images and videos of various particle types inside cells, both tracked and characterized, followed by a description of the LodeSTAR-model.

In the last section, he outlined the proposed continuation of his PhD, with an ongoing project for monitoring lipid droplets during long timescales and a neural network for 3D rotation parameter estimation of rotating biological samples.

PhD Student: Fredrik Skärberg
Supervisor: Daniel Midtvedt
Co-supervisors: Giovanni Volpe, Fredrik Höök

Fredrik Skärberg and audience in Nexus. (Photo by A. Ciarlo.)